Choosing a concentration for Lipidic Bicelles
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Is there a way to choose percentage for bicelles?...2 v. 4 --would be good to not have to use 4X protein at 4 concentrations.
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That's a good question. Since the final bicelle percentage for the crystallized proteins ranges from 2% to 8% (as shown in slide - "Membrane Proteins Crystallized in Bicelles"), it is ideal to test as many bicelle percentages as possible. That being said, I know the challenges involved in purifying large quantities of membrane proteins, so if there was only enough protein to test two percentages, I would recommend doing the 35% and the 25% - that way you've covered a higher and a lower percentage. And if one could do only single percentage, I would recommend the 35%.
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