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» Nucleosides, Nucleotides and their Analogs» Modified Nucleotides» Fluorescent Nucleotides» Intrinsically Fluorescent Nucleotide Analogs
Intrinsically Fluorescent Nucleotide Analogs
The Jena Bioscience compact, low molecular weight intrinsically fluorescent nucleotides combine fluorescence with close mimicry of the properties of natural nucleotides in respect of protein binding and interaction. They display environmentally sensitive fluorescence and therefore give strong signals upon a binding event which makes them perfect probes for stopped-flow and equilibrium analysis. The Mant-fluorophore is a commonly used FRET-probe in combination with Tryptophan or Tyrosin residues of the protein of interest.
| Fluorescent analog |
λabs | λem | εmax | Notes/Advantages |
| [nm] | [nm] | [l/(mol*cm)] | ||
| 2',3'-TNP... | 470 | 552 | 18.500 | Historically the first fluorescent analogs used as substrates for G-proteins and ATP binding proteins. Also available as pyrimidine probes. |
| 2',3'-Mant... | 355 | 448 | 5.800 | "THE CLASSICS" for small GTPases, heterotrimeric G-proteins, GPCRs, and motor protein kinetics. Small MW and environmentally sensitive fluorescence. FRET-probe for protein-intrinsic Tyr and Trp residues. |
| 2',3'-Ant... | 332 | 423 | 4.600 | Substitute for Mant-probes. Specific for tubulin, dynein, CaM, and disulfide isomerase. FRET-probe for protein-intrinsic Tyr and Trp residues. |
| ε-ATP... | 275 | 415 | 6.000 | Commonly used substrate of motor proteins, kinases, and other ATP binding proteins. |
| 2-Aminopurine- riboside- triphosphate |
320 | 370 | 6.000 | Fluorescent analog of ATP and/or GTP. |
Selected References:
- Gromadski et al. (2004) Nature Struct. & Molec. Biol. 11:316.
- Gille et al. (2004) NS Arch. Pharmacol. 369:141.
- Hiratsuka (2003) Eur. J. Biochem. 270:3479.
- Gille et al. (2003) NS Arch. Pharmacol. 368:210.
- Bagshaw (2001) J. Cell Sci. 114:459.
- Jameson et al. (1997) Methods Enzymol. 278:363 and references therein.
1 Unit = 1 µl of a 10 mM solution.
