Categories
Memb-PASSMemX Lipidic BicellesJANSi UVEX UV Imaging MicroscopesGlycon DetergentsMacromolecular CrystallographyNucleosides, Nucleotides and their AnalogsDownloadsPolicies
Site Information
Cart Contents
+1.888.901.7845
Home
» Macromolecular Crystallography» Initial Screening» Crystallization Screens» JBScreen Pentaerythritol
JBScreen Pentaerythritol
 JBScreen Pentaerythritol has been designed for efficient crystallization screening of biological macromolecules based on pentaerythritol polymers as precipitants. The screen was developed by Ulrike Demmer from the Max-Planck-Institute for Biophysics in Frankfurt. The choice of a suitable precipitant is of crucial importance for the crystallization of proteins. JBScreen Pentaerythritol utilizes two novel precipitating agents, i.e. pentaerythritol propoxylate and pentaerythritol epoxylate. Both are branched polymers containing a pentaerythritol backbone. Thus they differ from more traditional precipitants like MPD and PEG’s in size and nature. In addition, pentaerythritol polymers function as cryoprotectants. Protein crystals grown in high concentrations of these precipitants can be frozen directly from the crystallization drop. The successful application of pentaerythritol polymers to yield protein crystals was first described by Gulick et al. |
 JBScreen Pentaerythritol comprises of 96 unique conditions, based on 4 different pentaerythritol polymers as precipitating agent:
The advantage of JBScreen Pentaerythriol not only lies in the novel 96 conditions but also in the systematic arrangement of the unique reagents, which enables the user to compare individual conditions directly. Even if initial screening may not always yield crystals, valuable information about the protein under investigation can be obtained from the scoring sheet. |
 JBScreen Pentaerythritol Scoring Sheet |
All JBScreen Pentaerythritol screening kits include a detailed production report and data sheets. References Gulick et al. (2002) Pentaerythritol propoxylate: a new crystallization agent and cryoprotectant induces crystal growth of 2-methylcitrate dehydratase. Acta Cryst. D58:306. |
Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link. |
| Page 1 of 1 | 1 |
|
SKU: CS-210S
|
|
 |
|
|
SKU: CS-210L
|
|
|
|
|
SKU: CS-195
|
|
|
|
|
SKU: CS-194
|
|
|
|
|
SKU: CS-193
|
|
|
|
|
SKU: CS-192
|
|
|
|
|
SKU: CS-191
|
|
|
|
| Page 1 of 1 | 1 |
