Memb-PASS™

  Membrane Protein Crystallography

* Faster * Use Less Protein * Fully Exchange Detergents!

Use less protein and accelerate the process of crystallizing Membrane Proteins using the new, patent-pending Memb-PASS™ from Omscientia.  Dr. Wiener, and his laboratory at the University of Virginia, have developed and licensed to Omscientia, the Memb-PASS (Prompt Assay of Size and Stability) method for Membrane Protein Crystallography.  Memb-PASS starts with a microplate-based (Memb-PASS High and Low MWCO Plates ) detergent screening method that rapidly characterizes the stability and size of membrane proteins exchanged into a large panel of ninety-four carefully selected, unique, non-denaturing detergents chosen to suit a wide range of proteins:  Memb-PASS Differential Filtration Detergent Assay.  The DFDA is also useful for protein complexes since if a complex is formed, it would appear larger in the assay.  The entire assay utilizes sub-milligram quantities of membrane protein, and requires only several hours to perform while quickly helping you select the best detergent complex for your membrane protein!

Additionally licensed to Omscientia is a matched set of pre-crystallization solubility and crystallization screens:  Memb-PASS Solubility Screen and Memb-PASS Crystallization Screen.  After the protein is in a suitable detergent, as determined by the Memb-PASS Differential Filtration Assay, the Memb-PASS Solubility Screen, with twenty-four-conditions, is used to determine the proper protein concentration for crystallization.  

Once at proper concentration, the protein can then be set up for crystallization with the Memb-PASS Crystallization Screen, matched specifically to the Memb-PASS Solubility Screen and Memb-PASS Differential Filtration Detergent Assay.  The Memb-PASS Crystallization Screen is an innovative, ninety-six-condition screen, developed for integral membrane protein crystallization based on conditions that resulted in successful crystallization of membrane proteins.  The Memb-PASS methods are specifically designed to work together to help you significantly reduce the amount of protein, reagents and time spent on the crystallization of Membrane Proteins.
Patent Pending

*   Rapidly take your membrane protein target through the detergent selection
     process
*  Quickly ascertain suitable solubility conditions for your target (PDC)
*  Determine sizing information regarding your PDC in solution avoiding
     detergent micelle problems
*  Use micrograms of protein versus milligrams
*  Obtain results of 94 unique, membrane protein specific detergent conditions in a fraction
     of the time versus current methods
*  Method FULLY exchanges the detergent versus just dilution increasing success in crystallization 
*  Detergent concentrations tailored specifically for each detergent increasing effectiveness
      of detergents and reliability of results
*  Memb-PASS system is designed to work together yielding optimal results and eliminating
      the guess work of solubility and crystallization screening
*  Memb-PASS Crystallization Screen reduces the number of crystallization experiments necessary
      to obtain diffraction quality crystals of membrane proteins

Memb-PASS Step-by-Step Method Diagrams

Learn about Memb-PASS from our FREE Webinar

References
Vergis JM, Purdy MD, Wiener MC. (2010 Dec 1) A high-throughput differential filtration assay to screen and select detergents for membrane proteins. Anal. Biochem. Dec 2010.  407(1):1-11.  Epub 2010 Jul 25.

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