Membrane Protein Crystallography
Use less protein and accelerate the process of crystallizing Membrane Proteins using the new, patent-pending Memb-PASS™ from Omscientia. Dr. Wiener, and his laboratory at the University of Virginia, have developed and licensed to Omscientia, the Memb-PASS (Prompt Assay of Size and Stability) method for Membrane Protein Crystallography. Memb-PASS starts with a microplate-based (Memb-PASS High and Low MWCO Plates ) detergent screening method that rapidly characterizes the stability and size of membrane proteins exchanged into a large panel of ninety-four carefully selected, unique, non-denaturing detergents chosen to suit a wide range of proteins: Memb-PASS Differential Filtration Detergent Assay. The DFDA is also useful for protein complexes since if a complex is formed, it would appear larger in the assay. The entire assay utilizes sub-milligram quantities of membrane protein, and requires only several hours to perform while quickly helping you select the best detergent complex for your membrane protein!
Additionally licensed to Omscientia is a matched set of pre-crystallization solubility and crystallization screens: Memb-PASS Solubility Screen and Memb-PASS Crystallization Screen. After the protein is in a suitable detergent, as determined by the Memb-PASS Differential Filtration Assay, the Memb-PASS Solubility Screen, with twenty-four-conditions, is used to determine the proper protein concentration for crystallization.
Once at proper concentration, the protein can then be set up for crystallization with the Memb-PASS Crystallization Screen, matched specifically to the Memb-PASS Solubility Screen and Memb-PASS Differential Filtration Detergent Assay. The Memb-PASS Crystallization Screen is an innovative, ninety-six-condition screen, developed for integral membrane protein crystallization based on conditions that resulted in successful crystallization of membrane proteins. The Memb-PASS methods are specifically designed to work together to help you significantly reduce the amount of protein, reagents and time spent on the crystallization of Membrane Proteins.
* Rapidly take your membrane protein target through the detergent selection
* Quickly ascertain suitable solubility conditions for your target (PDC)
* Determine sizing information regarding your PDC in solution avoiding
detergent micelle problems
* Use micrograms of protein versus milligrams
* Obtain results of 94 unique, membrane protein specific detergent conditions in a fraction
of the time versus current methods
* Method FULLY exchanges the detergent versus just dilution increasing success in crystallization
* Detergent concentrations tailored specifically for each detergent increasing effectiveness
of detergents and reliability of results
* Memb-PASS system is designed to work together yielding optimal results and eliminating
the guess work of solubility and crystallization screening
* Memb-PASS Crystallization Screen reduces the number of crystallization experiments necessary
to obtain diffraction quality crystals of membrane proteins
Memb-PASS Step-by-Step Method Diagrams
Learn about Memb-PASS from our FREE Webinar
Vergis JM, Purdy MD, Wiener MC. (2010 Dec 1) A high-throughput differential filtration assay to screen and select detergents for membrane proteins. Anal. Biochem. Dec 2010. 407(1):1-11. Epub 2010 Jul 25.
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|Memb-PASS MWCO Differential Filtration Plates|
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