Memb-PASS Crystallization Screen™

  Membrane Protein Crystallography

* Increase Success in Membrane Protein Crystallization

Use less protein and accelerate the process of crystallizing Membrane Proteins using the patent-pending Memb-PASS Screening Method and Kits from Omscientia by significantly reducing the number of crystallization experiments necessary to obtain crystals of membrane proteins suitable for X-ray diffraction.  Matched to the Memb-PASS Solubility Screen, the Memb-PASS Crystallization Screen is an innovative 96-condition screen, developed and tuned for integral membrane protein crystallization.  The Memb-PASS Crystallization screen is based on conditions that resulted in successful crystallization of membrane proteins.  The combined use of these screens can increase the rate of successful crystallization for membrane proteins and has significantly outperformed current commercially available membrane protein crystallization screens.

Dr. Wiener, and members of his laboratory at the University of Virginia, have developed, and licensed to Omscientia, the Memb-PASS method for Membrane Protein Crystallography.  Memb-PASS (Prompt Assay of Size and Stability) is a new, patent-pending, protein-efficient method to rapidly screen your integral membrane proteins for proper detergent selection in the preparation for crystallization trials.  Also useful for soluble proteins, the Memb-PASS Differential Filtration Detergent Assay allows for a quick and robust assessment of membrane protein stability and rudimentary sizing information in a high-throughput format while quickly helping you select the best detergent complex for your membrane protein. 

The Omscientia Memb-PASS Solubility Screen and Omscientia Memb-PASS Crystallization Screen are specifically designed to work together with the Differential Filtration Detergent Assay and help you significantly reduce the amount of protein, reagents and time spent on the crystallization of Membrane Proteins.  After the protein is in a suitable detergent, as determined by the Memb-PASS Differential Filtration Detergent Assay, the Memb-PASS Solubility Screen, with 24-conditions, is used to determine the proper protein concentration for crystallization.  Once at proper concentration, the protein can then be set up for crystallization with the Memb-PASS Crystallization screen.
Patent Pending

*  Memb-PASS crystallization screen is based on conditions that resulted in
      successful crystallization of membrane proteins
*  Tuned for Integral Membrane Protein Crystallization
*  Memb-PASS system is designed to work together yielding optimal results and eliminating
      the guess work of solubility and crystallization screening
*  Memb-PASS Crystallization Screen reduces the number of crystallization experiments necessary
      to obtain diffraction quality crystals of membrane proteins

Memb-PASS Step-by-Step Method Diagrams

Learn about Memb-PASS from our FREE Webinar

  Download Crystallization Screen Conditions

References
Vergis JM, Purdy MD, Wiener MC. (2010 Dec 1) A high-throughput differential filtration assay to screen and select detergents for membrane proteins. Anal. Biochem. Dec 2010.  407(1):1-11.  Epub 2010 Jul 25.

Memb-PASS Crystallization Screens: