Memb-PASS Differential Filtration
  Detergent Assay™

  Membrane Protein Crystallography

 
* Screen 94 Unique Detergents * Use Less Protein
* Faster * Fully Exchange Detergents!

Quickly ascertain size and stability with use of the Memb-PASS Differential Filtration Detergent Assay and the Memb-PASS High and Low MWCO Plates in hours using micrograms of protein!  Dr. Wiener, and members of his laboratory at the University of Virginia, have developed, and licensed to Omscientia, the Memb-PASS method for Membrane Protein Crystallography.  Memb-PASS (Prompt Assay of Size and Stability) is a new, patent-pending, protein-efficient method to rapidly screen your integral membrane proteins for proper detergent selection in the preparation for crystallization trials.  Also useful for soluble proteins, the Memb-PASS Differential Filtration Detergent Assay allows for a quick and robust assessment of membrane protein stability and rudimentary sizing information in a high-throughput format while quickly helping you select the best detergent complex for your membrane protein.  Memb-PASS starts with a microplate-based detergent screening method that rapidly characterizes the stability and size of membrane proteins exchanged into a large panel of ninety-four carefully selected and unique detergents that feature non-denaturing detergents chosen to suit a wide range of proteins.  The DFDA is also useful for protein complexes since if a complex is formed, it would appear larger in the assay.  The entire assay utilizes sub-milligram quantities of membrane protein, and requires only several hours to perform! 
Patent Pending

*   Rapidly take your membrane protein target through the detergent selection process
*  Quickly ascertain suitable solubility conditions for your target (PDC)
*  Determine sizing information regarding your PDC in solution avoiding detergent micelle problems
*  Use micrograms of protein versus milligrams
*  Obtain results of 94 unique, membrane protein specific detergent conditions in a fraction
      of the time versus current methods
*  Method FULLY exchanges the detergent versus just dilution increasing success in crystallization 
*  Detergent concentrations tailored specifically for each detergent increasing effectiveness
      of detergents and reliability of results
*  Memb-PASS system is designed to work together yielding optimal results and eliminating
      the guess work of solubility and crystallization screening

Memb-PASS Step-by-Step Method Diagrams

Learn about Memb-PASS from our FREE Webinar 

  Download DFA Conditions

References
Vergis JM, Purdy MD, Wiener MC. (2010 Dec 1) A high-throughput differential filtration assay to screen and select detergents for membrane proteins. Anal. Biochem. Dec 2010.  407(1):1-11.  Epub 2010 Jul 25.

Memb-PASS Differential Filtration Detergent Screens: