Memb-PASS Solubility Screen™

  Membrane Protein Crystallography

* Determine the Proper Protein Concentration for Crystallization

The patent-pending Memb-PASS Solubility Screen and Memb-PASS Crystallization Screen are specifically designed to work together with the Memb-PASS Differential Filtration Detergent Assay.  After the protein is in a suitable detergent, as determined by the Memb-PASS Differential Filtration Detergent Assay, the Memb-PASS Solubility Screen, with 24-conditions, is used to determine the proper protein concentration for crystallization.  Once at proper concentration, the protein can then be set up for crystallization with the Memb-PASS Crystallization screen, developed and tuned for integral membrane protein crystallization.  The solubility pre-screen is also extremely useful as a follow-up to the Differential Filtration Detergent Assay, because the appropriate concentration of the protein for crystallization may be very different in different "good" detergents identified with the detergent screen.  Significantly reduce the amount of protein and number of crystallization experiments necessary to obtain crystals of membrane proteins suitable for X-ray diffraction experiments with Memb-PASS.
Patent Pending

*  Quickly ascertain suitable concentration for your target protein
*  Tuned for Integral Membrane Protein Crystallization
*  Memb-PASS system is designed to work together yielding optimal results and eliminating
      the guess work of solubility and crystallization screening
*  Memb-PASS Crystallization Screen reduces the number of crystallization experiments necessary
      to obtain diffraction quality crystals of membrane proteins

Memb-PASS Step-by-Step Method Diagrams

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  Download Solubility Screen Conditions

References
Vergis JM, Purdy MD, Wiener MC. (2010 Dec 1) A high-throughput differential filtration assay to screen and select detergents for membrane proteins. Anal. Biochem. Dec 2010.  407(1):1-11.  Epub 2010 Jul 25.

Memb-PASS Solubility Screen: